The non-conversion rate of each sample was obtained by analysing the rate of methylated cytosine (mCs) in. Note. Introduction. 2-Methylpyridine - borane (1:1) Molecular Formula CHBN. Average mass 124. 2. This format is originally defined by methylpy, a python. 在第一步过程中，smoothing可以更好帮助估算甲基化（针对whole-genome BS-seq）。. 2-Acetyl-6-Methylpyridine. @rbpisupati even after manual loading of bowtie along with default bowtie2 with methylpy . Predicted data is generated using the US Environmental Protection Agency s EPISuite™. 137161 Da. Here, we characterized global epigenomic changes during the direct reprogramming of mouse fibroblasts to. MethylC-seq: analysis was performed by Methylpy 1. 95 Boiling Pt, Melting Pt, Vapor Pressure Estimations (MPBPWIN v1. Supplementary_files_format_and_content: tab delimited allC file showing total number of reads (total) and reads reporting methylation (mc. I encountered some problems when running run_test. 4 , call-methylation-state and merged both strand to increase coverages at each CG sites Differentially methylated regions (DMRs) were defined by the DMRfind function in methylpy 1. NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data. 00. 0 and. 01 for each DMSs. Predicted data is generated using the US Environmental Protection Agency s EPISuite™. Product Certifications Promotions. We retained bins if they contain. Alkylpyrazine. In addition, full historical log of module additions and. 2) with "methylpy allc-to-bigwig --bin-size 10" genome build: hg38{"payload":{"allShortcutsEnabled":false,"fileTree":{"":{"items":[{"name":"bin","path":"bin","contentType":"directory"},{"name":"docs","path":"docs","contentType. Welcome to the home page of methylpy, a pyhton-based analysis pipeline for (single-cell) (whole-genome) bisulfite sequencing data (single-cell) NOMe-seq data; differential methylation analysis; methylpy is available at github and PyPI. Saved searches Use saved searches to filter your results more quickly python run_test. 1. The biotransformations of gamma-picoline were investigated in the rat. 3. Dear author: Thank you for develop such a useful tools for WGBS. The genome is converted into a forward strand reference (all Cs to Ts) and a reverse strand reference (all Gs to As). I ran methylpy for multiple samples, and then tried to reidentify DMRs in a subset of those samples. {"payload":{"allShortcutsEnabled":false,"fileTree":{"":{"items":[{"name":"bin","path":"bin","contentType":"directory"},{"name":"docs","path":"docs","contentType. 2) with "methylpy allc-to-bigwig --bin-size 10" genome build: hg38Per-cytosine methylation level was obtained using methylpy (version 1. 4. 42-Methoxy-6-methylpyrazine. Methylpy does not calculate group-wise difference but it is no super difficult to calculate statistics like difference in median/mean methylation level using R or python pandas. After alignment of the sequence reads to a reference genome, methylation counts are analyzed to determine genomic regions that are differentially methylated between two or. Closed. 163 Da. Compared to single-cell transcriptomic data, which carry information on the transcriptional state, DNA methylome data carry information on mechanisms of gene expression regulation, such as the involvement of specific cis-regulatory elements and their interactions. 2) with "methylpy allc-to-bigwig --bin-size 10" genome build: hg38 {"payload":{"allShortcutsEnabled":false,"fileTree":{"":{"items":[{"name":"bin","path":"bin","contentType":"directory"},{"name":"docs","path":"docs","contentType. 利用DMLtest函数call DML，分为一下几个步骤：. In addition to comparing our own sequencing files, we also used methylpy to analyze the documented data (all from plants: Oryza sativa). 250bp is arbitrary to some degree but it can be justified by the observation that the methylation status of CpGs within ~200bp are correlated. Monoisotopic mass 171. Average mass 198. 2. For MethylC-seq data: Reads were trimmed, aligned, and methylation called using the methylpy pipeline (Schultz et al. 560 Da. Molecular Formula CHNO. jar Traceback (most recent call last): File "/home/poch. rapa and ~6. $25. 100052 Da. Although many organic electrode materials had been studied before, only a few polymers showed reliable redox reversibility and cycling stability. By default, methylpy does not do the test to speed up processing and just places 1s in the last. ***> wrote: Hello yupenghe, The alignment worked with a subset of 1million reads. Pull requests. 2-Methyl-3-propylpyrazine. DNA methylation, where cytosine bases are methylated by distinct molecular pathways in either the CG, CHG, or CHH (H = C, A, or T) sequence contexts, is a. Supplementary_files_format_and_content: tab delimited allC file showing total number of reads (total) and reads reporting methylation (mc. 1 HiC-seq: analysis was performed by HiC-pro. Tab-delimited (allc) files containing methylation level for every cytosine positions was generated using methylpy call_methylated_sites function (Schultz et al. Part 4: Development of 3-[3-tert-Butylsulfanyl-1-[4-(6-ethoxypyridin-3-yl)benzyl]-5-(5-methylpy. {"payload":{"allShortcutsEnabled":false,"fileTree":{"docs":{"items":[{"name":"images","path":"docs/images","contentType":"directory"},{"name":"README. tsv allc_GSM2481629. Star 124. Supplementary_files_format_and_content: tab delimited allC file showing total number of reads (total) and reads reporting methylation (mc. 1. 68, but the latter is labeled as. This. dide. ChemSpider ID 2043567. For example, ->. tsv allc_GSM2986379. . 3 RNA-seq: analysis was performed by hisat2 v2. Supplementary_files_format_and_content: tab delimited allC file showing total number of reads (total) and reads reporting methylation (mc. - importing call_mc_se: pass - importing call_mc_pe: pass - importing DMRfind: pass - importing parser: pass Check methylpy executable:pass methylpy is successfully installed!The ALLC (ALL Cytosine) format is a tab-separated table containing base level methylation and coverage counts. We found that when methylpy was used in plant genomes, the methylation rate was significantly reduced, about half. - GitHub - jlab-code/MethylStar: A fast and robust pre-processing pipeline for bulk or single-cell whole-genome bisulfite sequencing (WGBS) data. ChemSpider ID 2043567. 0 and. PCR plate was treated with the following program: 95 °C for 2 min, 98°C for 30 sec, 17 cycles of (98°C for 15 sec, 64°C for 30 sec, 72°C for 2min), 72°C for 5 min then 4°C. The files are located here. Background DNA methylation is an epigenetic modification that is studied at a single-base resolution with bisulfite treatment followed by high-throughput sequencing. 3b ChIP-seq: analysis was performed by bowtie1. Fig. 1. 0 and. Usage on Bridges-2. I called number of procs as follows methylpy add-methylation-level --input-tsv-file /xx/xx/_short_window1h. Libraries were single-end 50, 75, or 150 bp. 3b ChIP-seq: analysis was performed by bowtie1. Methylpy used bowtie2 to map reads to forward and reversed strand and then combine the results. 42): Boiling Pt (deg C): 326. 6. For MethylC-seq data: Reads were trimmed, aligned, and methylation called using the methylpy pipeline (Schultz et al. Identification of differential interaction regions. Single-nucleus DNA methylation sequencing was recently used to classify neuronal populations in the human and mouse brain, including multiple subtypes of excitatory and inhibitory neurons with distinct cell type–specific epigenetic signatures (). Molecular Formula CHClN. For methylpy we will need to use the --pbat option. Only DMRs with significant differences (P ≤ 0. py) to convert them to CGmap. gz PSMC_inference, wgs for 19 California Q. Monoisotopic mass 136. 2,6-Di-tert-butyl-4-methylphenol is used as an antioxidant for cosmetics, vitamins, pharmaceuticals, rubber, oils and fats. 1. 2) “call-methylation-state” tool with “--binom-test True --paired-end True” options CG methylation levels bigwig files were generated with methlypy (version 1. 4). Monoisotopic mass 107. gz, including CX report files generated by Bismark, the methylation calls generated by methratio. MethylC-seq: analysis was performed by Methylpy 1. Status: Public on Jun 17, 2015: Title: MethylC-seq_VIP_neurons_rep1: Sample type: SRA : Source name: Vasoactive intestinal peptide-positive inhibitory neurons from mouse neocortex ChemicalBook 致力于为化学行业用户提供2,6-二叔丁基吡啶的性质、化学式、分子式、比重、密度,同时也包括2,6-二叔丁基吡啶的沸点、熔点、MSDS、用途、作用、毒性、价格、生产厂家、用途、上游原料、下游产品等信息。 Welcome to the home page of methylpy, a pyhton-based analysis pipeline for (single-cell) (whole-genome) bisulfite sequencing data (single-cell) NOMe-seq data; differential methylation analysis; methylpy is available at github and PyPI. Additionally, the analysis results from the Methylation Array Harmonization Workflow are expected to be of higher quality than results. We would like to show you a description here but the site won’t allow us. 1 DAP-seq: analysis was performed by bowtie2. Molecular Formula CHNO. 67 estimate) = 1. 079315 Da. 3b ChIP-seq: analysis was performed by bowtie1. Herein, a high-performance dual-ion battery system is proposed, which consists of a graphite cathode and SnS2 anode, with a high-concentration lithium salt electrolyte (4 M LiTFSI). The material consists of a cis/trans-isomer mixture. 2 with default parameters. 01 for each DMSs. 1. Similarly bismark also has the --pbat option to handle this data. py in BSMAP (v2. 163 Da. 1. Electrochemical energy sources support the growth in performance and portability of consumer electronics. 67 estimate) = 1. /YOUR/TMP/DIR/). 571 Da. 2-amino-5-isopentyl-6-methylpyrimidin-4-ol. md","path":"docs. Raw reads were trimmed for adapters and mapped using methylpy to all the amplicons pooled together for sequencing. Methylprep is part of the methylsuite. 1. Methylpy . 0 and. tsv P4-HT_pool. when I run the following script I get IOError: [Errno 71. 2,6-DI-Tert-butyl-4-methoxyphenol is a natural product found in Betula platyphylla var. 8 and GEM v2. Genome_build: Taeniopygia guttata v3. 2-Acetyl-5-methylpyridine. Raw reads were trimmed for adapters and mapped using methylpy to all the amplicons pooled together for sequencing. Methylpy accounts for this non-conversion in calling methylated sites. The electrostatic interaction, which is the strongest interaction between a solvent and the ions, is. tests for DMRfind and reidentify-DMR are failedWe then performed differential methylated regions (DMR) analysis using the “methylpy DMRfind” function among all clusters, as previously described 68. NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data. 063660 Da. 67 estimate) = 1. Hi, I am using the methylpy to add methylation levels. Hill, Maria-Jesus Blanco, Francesco G. Average mass 127. --- layout: post title: Aug. Sequencing quality was verified by FastQC and clean reads were subsequently mapped to the A. Average mass 106. 560 Da. ChEBI. 3. 5 ATAC-seq: analysis was performed by bowtie1. We review the synthesis routes, conductivity enhancement methods and applications of PEDOT in industrial and biomedical. More relevant content may be added as customer demand. Differential methylation in Geoduck juveniles reared in different CO2 conditions - Shelly_Pgenerosa/destranded_v_stranded. It is used as a fuel additive in the petroleum industry and also used in hydraulic fluids, turbine and gear oils and jet fuels. He intensively worked with the DNA methylation involved in germ cell and embryo development. It is totally fine to set the last column to be 1. methylpy. . start: required. py : Tue Nov 12 15:47:14 2019 Test importing methylpy module. This article summarises the industrial applications of poly-(3,4-ethylenedioxythiophene) (PEDT, PEDOT). Methylpy is a python-based analysis pipeline for (single-cell) (whole-genome) bisulfite sequencing data (single-cell) NOMe-seq data; differential methylation analysis; Documentation. io/biocontainers/methylpy. 013794 Da. Per-cytosine methylation level was obtained using methylpy (version 1. Request PDF | 5-Lipoxygenase-Activating Protein (FLAP) Inhibitors. Molecular Formula CHNO. More relevant content may be added as customer demand increases. - importing call_mc_se: pass - importing call_mc_pe: pass - importing DMR. Product Certifications. Supplementary_files_format_and_content: tab delimited allC file showing total number of reads (total) and reads reporting methylation (mc. 1 HiC-seq: analysis was performed by HiC-pro v2. Individual CG methylation state is processed with methylpy 1. Everything looks good when I run the run_test. The genome is converted into a forward strand reference (all Cs to Ts) and a reverse strand reference (all Gs to As). 21-26, 2019 Geoduck genome paper-DMRs category: Geoduck epigenomics --- ## Aug. methylpy has took long time. This allows to unambiguously reveal that the Ueff mainly depends on the. We. methylpy DMRfind --allc-files WT_ht_pool. When I reidentify DMRs, I found some cases where the methylation levels do not correlate with the DMR labels. Molecular Formula CHClN. 5 ATAC-seq: analysis was performed by bowtie1. Dual-ion batteries are known for anion storage in the cathode coupled to cation incorporation in the anode. [bam_sort_core] merging from 0 files and 8 in-memory blocks. methylpy . Similarly bismark also has the --pbat option to handle this data. DMRfinnd RMS issue #49. 055389 Da. Alternatively, we can run cutadapt manually and then run methylpy with trimming step turned off. 2 shows a spectrum 1H-NMR of the product of formula (I) obtained according to the process of the present invention; (0010 FIG. 1 HiC-seq: analysis was performed by HiC-pro v2. /Allc/ICE2-CR-3. 50-242-5786. Human body epigenome maps reveal noncanonical DNA methylation variation Nature 523, 212–216 (2015). For cell-type identification, only mCG. PEDT applications are reviewed depVisit ChemicalBook To find more 2-Methoxy-3-methylpyrazine(2847-30-5) information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular weight, physical properties,toxicity information,customs codes. methylpy. Interestingly, although gemBS was the fastest pipeline for the A. 3 RNA-seq: analysis was performed by hisat2 v2. ChemSpider ID 21423254. 1 Supplementary_files_format_and_content: <chromosome> <cytosine position, 1-index> <strand> <CNN (N = A, T, C, G)> <methylated cytosines at this site> <mapped cytosines at this site> <binomial test;1 means methylated, 0 means. 1 HiC-seq: analysis was performed by HiC-pro v2. 8. 1. The allC file generated by methylpy was used to calculate methylation percentage for a locus. For MethylC-seq data: Reads were trimmed, aligned, and methylation called using the methylpy pipeline (Schultz et al. 5 ATAC-seq: analysis was performed by bowtie1. Briefly, soy-We would like to show you a description here but the site won’t allow us. Methylpy software was used to extract weighted methylation rate at each available cytosine from BAM files containing only deduplicated and uniquely mapped reads (Schultz et al. tsv. thaliana reference genome index sequence version 10 (TAIR10) using Methylpy software . Fig. In this review, we introduce a conducting polymer called poly(3,4-ethylenedioxythiophene) (PEDOT), due to its interesting features such as satisfactory conductivity, good transparency, easy processability, low price, small redox potential and good electrochromic properties. 4 by joining at least two DMSs within 250bp, FDR < 0. The allC file generated by methylpy was used to calculate methylation percentage for a locus. 5 and StringTie v1. Another pipeline, MethylPy [109], is similar to MethylStar in its contents; however, MethylStar was able to perform the preprocessing step for 100 single cells in 2225 min, compared to MethylPy. The allC file generated by methylpy was used to calculate methylation percentage for a locus. During the charging process, Pyr 14 + cations combine with NTCDA, and TFSI-anions insert into the graphite layers. System Variables. 4. <xarray. This product was recently added by customer request, and is available for your convenience. Differentially methylated regions (DMRs) were called by DMRfind in MethylPy for CG context with the option --min-num-dms 5 (at least five differentially methylated sites). Postdoctoral Fellow. Hi, I am working with WGBS data on a non-model organism. ; Once the image has been created successfully, run the container using the following command. , 2015). methylpy is available at github and PyPI. 3. Supplementary_files_format_and_content: tab delimited allC file showing total number of reads (total) and reads reporting methylation (mc. 3. 25 Boiling Pt, Melting Pt, Vapor Pressure Estimations (MPBPWIN v1. Hi yupenghe, Can you look into the bam-quality-filter program? It does not function as its supposed to. I used methylpy to generate the allC files and then merge replicates for samples -all of w. This product was recently added by customer request, and is available for your convenience. ChemSpider ID 146179. Saved searches Use saved searches to filter your results more quicklyEnergy issues have attracted great concern worldwide. Average mass 434. 4. I have run a methylpy pipeline for one month at least. g. A fast and robust pre-processing pipeline for bulk or single-cell whole-genome bisulfite sequencing (WGBS) data. HPC_METHYLPY_DIR - installation directory Differentially methylated regions (DMRs) were called by DMRfind in MethylPy for CG context with the option --min-num-dms 5 (at least five differentially methylated sites). e. 30 Boiling Pt, Melting Pt, Vapor Pressure Estimations (MPBPWIN v1. S7 Fig: The allelic effects of AGO1 in the RdDM pathway and the similarity to AGO1 activity. 5) or a related hydrocarbon. #83 opened on Feb 20 by Biomamba. 6. 2) “call-methylation-state” tool with “--binom-test True --paired-end True” options CG methylation levels bigwig files were generated with methlypy (version 1. . - importing call_mc_se: pass - importing call_mc_pe: pass - importing DMR. This will require you to cd into the environment located in your anaconda (or miniconda) directory. 4. 1 DAP-seq: analysis was performed by bowtie2. 42): Boiling Pt (deg C): 329. methylpy . region_id: optional, but recommended to have. For methylpy we will need to use the --pbat option. 1038/nprot. 1 shows the synthesis scheme 1-(6-methylpy ridin-3-yl)-2-4-(methylsulfonyl)phenylethanone according to the preferred aspects of the present invention; 0009 FIG. Molecular Formula CHN. Robichaud. You may want to set the TMPDIR environmental variable to the (absolute) path of a directory on hard drive with sufficient space (e. 1. Version 1. Everything looks good when I run the run_test. ChemSpider ID 108608. Developing new energy has been the main choice, and the exploitation of the electrochemical energy storage devices plays an important role. Homepage: License: APACHE / Apache 2. Genome_build: mm10. 01) and absolute differences greater than 0. Schultz; beta version of tutorial is released! What can methylpy do? Processing bisulfite sequencing data and NOMe-seq data. 8 and GEM v2. Herein, a high-performance dual-ion battery system is proposed, which consists of a graphite cathode and SnS2 anode, with a high. 014130 Da. You can then perform annotation, motif scan and further analysis using the methods described in the following sections. Can you tell me if my pipeline is finishe. Schultz; beta version of tutorial is released! What can methylpy do? Processing bisulfite sequencing data and NOMe-seq data. For example, ->. Harrison, Ethan C. NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data. Genome_build: Populus trichocarpa Stettler 14 v1. Check our Scrabble Word Finder, Wordle solver, Words With Friends cheat dictionary, and WordHub word solver to find words starting with methylpy. 2-Fluoro-4-methylpyridine-5-bo. yupenghe / methylpy Public. . thaliana was used as an unmethylated control. 2) “call-methylation-state” tool with “--binom-test True --paired-end True” options CG. 2-Acetyl-4-methylpyridine. Poly(3,4-ethylenedioxythiophene)s are the conducting polymers (CP) with the biggest prospects in the field of bioelectronics due to their combination of characteristics (conductivity, stability. Note ; Version 1. NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data. 4. Supplementary_files_format_and_content: tab delimited allC file showing total number of reads (total) and reads reporting methylation (mc. 2) “call-methylation-state” tool with “--binom-test True --paired-end True” options CG methylation levels bigwig files were generated with methlypy (version 1. Methylpy software was used to extract weighted methylation rate at each available cytosine from BAM files containing only deduplicated and uniquely mapped reads (Schultz et al. 5 ATAC-seq: analysis was performed by bowtie1. 3 RNA-seq: analysis was performed by hisat2 v2. 1. dide. methylkit：用于高通量亚硫酸氢盐测序的DNA甲基化分析和注释的R包。. Methylpy used bowtie2 to map reads to forward and reversed strand and then combine the results. shpc-registry automated BioContainers addition for methylpy After installing shpc you will want to install this container module: $ shpc install quay. 1 Implementation. 2009. not masked) methylpy was initiated by and built on the work of Mattew D. Average mass 143. MethylC-seq: analysis was performed by Methylpy 1. The weighted average DNA methylation levels of specific regions of interest were calculated by the add-methylation-level function in MethylPy under the restriction (depth ≥ 5) and further. Methylpy . BMCGenomics (2020) 21:479 Page4of8 Table1TableshowingdifferentfeaturesofMethylStarascomparedtootherBS-seqpipelines Methylpy MethylStar methylseq gemBS. methylpy only considers cytosines that are in uppercase in the genome fasta file (i. Shahryaryetal. hello im having this issue. In addition, full historical log of module additions and. Learn more about Teamsmethylpy pipeline (Schultz etal. 1. 2,6-di-tert-butyl-4-methoxyphenol is a member of phenols and a member of methoxybenzenes. 2) “call-methylation-state” tool with “--binom-test True --paired-end True” options CG methylation levels bigwig files were generated with methlypy (version 1. Catalog No. I used Bismark (with Bowtie) to align my reads and would like to use Methylpy to identify DMRs. Closed. In other words, molecular interactions between the solvent and the ions increase from TEA + to BF 4 ¯. We compared results from mapping of the parental data to. 7 million hectares (James, 2016). Kazdoba, David Lanzetta, Michael Lewis, Michael C. 3. We utilized window strategy (Stroud etal. Methylpy home page . The methylation level of each cytosine was then computed with Methylpy v. 2011) is used to trim adaptor sequences and then bowtie (Langmead et al. tsv allc_GSM2986381. Or you can browse through the sub-categories or software package names in the table below. Click here to find the full list of applications that can be run on HiPerGator. The ALLCools workflow encompasses the following two sections: CELLULAR ANALYSIS: This section goes over basic clustering steps, differential methylated genes (DMG) analysis, data integration, and doublets identification; GENOMIC ANALYSIS: This section covers differential methylated region (DMR) calling, genome annotation, DNA motif analysis. Technical Service. 1. #85 opened on May 17 by FarzanehRah. 8. NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data. Cutadapt (Martin et al. 预测所有CpG位点的平均甲基化水平. Quirk, Albert J. 2. 10-20/21/22-36/38 Alfa Aesar H25772: 3 Alfa Aesar H25772: 9-23-26-36/37 Alfa Aesar H25772: DANGER: FLAMMABLE, irritates skin, eyes, lungs Alfa Aesar H25772: H226-H302-H312-H332-H315-H319 Alfa Aesar H25772: P210-P261-P303+P361+P353-P305+P351+P338-P403+P235-P501a Alfa Aesar H25772: Warning Alfa Aesar H25772:. 1. 008713 Da. To see what versions of methylpy are available and if there is more than one, which is the default, along with. Scatter plots show correlations of DML induced by NRPE1’ and mutants, nrpe1-11 and ago1, for each TE. 3. . Downgrading cutadapt to the version methylpy supported is one option. 该包的目的是处理RRBS以及WGBS的测序数据。. We review the synthesis routes, conductivity. 1 HiC-seq: analysis was performed by HiC-pro. Chloroplast genomic sequence from A. ChemicalBook 致力于为化学行业用户提供2,6-二叔丁基-4-甲基吡啶的性质、化学式、分子式、比重、密度,同时也包括2,6-二叔丁基-4-甲基吡啶的沸点、熔点、MSDS、用途、作用、毒性、价格、生产厂家、用途、上游原料、下游产品等信息。 Abstract. 3. tsv allc_GSM2986389. I ran methylpy 1. Two brands of normal saline, OpticareTM and Klean & KareTM, were used by 30 contact lens wearers on alternate days. I have problem with the first step building references. 2) “call-methylation-state” tool with “--binom-test True --paired-end True” options CG methylation levels bigwig files were generated with methlypy (version 1. 194 Da. 5 and StringTie v1. e. 2-Benzylamino-4-Methylpyridine. Dear Yupeng, I am having trouble with running DMRfind for some of my datasets that were downloaded from NCBI (Arabidopsis bisulfite seq GSE67216). Another pipeline, MethylPy , is similar to MethylStar in its contents; however, MethylStar was able to perform the preprocessing step for 100 single cells in 2225 min, compared to MethylPy, which took 5518 min . Interestingly, although gemBS was the fastest pipeline for the A. An ALLC file contains 7 mandatory columns. Welcome to the home page of methylpy, a pyhton-based analysis pipeline for (single-cell) (whole-genome) bisulfite sequencing data (single-cell) NOMe-seq data Note ; tutorial is being written ; In new version, allc files for one sample are no longer split and the current allc format does not support header.